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.Aqueous-phase microemulsions form cubic crystals with layered and layer-intercalated structures.
Ionizable surfactants like cetyltrimethylammonium bromide (CTAB) and dodecyltrimethylammonium bromide (DTMAB) induce the formation of cubic crystals instead of lamellar crystals from both aqueous microemulsions containing cationic and anionic surfactants. The cubic crystals are composed of lamellar bilayer sheets containing aqueous microemulsion cores. The formation of cubic crystals was confirmed by X-ray crystallography and was unaffected by the addition of neutral proteins like BSA and gamma-globulin, different polysaccharides, or sugars. Furthermore, the formation of cubic crystals was not affected by the addition of polyionic or zwitterionic surfactants. Cryo-transmission electron microscopy (TEM) images showed that the obtained cubic crystals are composed of bilayer sheets with regular interfacial spacing. By means of surface plasmon resonance (SPR), it was shown that the ionizable surfactant heads concentrate at the aqueous-lipid interface (microemulsion core). The analysis of the stability of the ordered lipid bilayer sheets toward compression showed a compression threshold of 0.19-0.23 mN m-1. In aqueous-phase microemulsions with added DNA the cubic crystals are formed. The formation of the cubic crystals is attributed to the screening effect of DNA molecules that avoid the long-range Coulomb repulsion of like charges.Title
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