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These tips, tricks, and techniques will help you create valuable images in Photoshop with a little practice. Use the following tip to create a simple Photoshop template that is usable for all future projects. * If you have multiple images you want to use in a project and each has its own naming convention, create one photo template for all of your images. Photoshop * Open Photoshop CS4 (Windows) or Elements 11 (Mac). * Choose File⇒New. * Choose File Type as Photoshop Photo. * Click Open. You now see an image that displays a tiny purple check mark icon; this is your project’s template image. * Click the brush icon with the black arrow pointing down. * Click the color swatch icon and choose any of the preset colors, like Blue Sky, Forest Leaves, or Golden. * Click the paint bucket. * Hold down the SHIFT key while you paint with the brush. * Paint the image with colors of your choice. * When you’re finished painting, click Save. The template now exists as your photo template. If you need to open it, simply open the folder in which it resides and double-click the photo. You can even load it from the template drop-down menu in the upper-right corner of the main Photoshop window. * The image you created is a simple photo template. It is not a web template. That’s because you’re not going to create an actual web design document using the Photoshop template. You’re going to use it for projects such as logo designs, business cards, or brochures. For a web template, create a web design document. The web design document has a much different file type. It has.webarchive extension. For more information on creating a web design document, flip to Chapter 6. * Click File⇒Save As. * Type a name for the file in the Save as Type box. * Change the Format to Photoshop.psd File. * Click Save. When you reopen the new file, you see the generic Photoshop.psd file opening in Photoshop. You can save an image as a Photoshop.psd file for future use in this method as well. For example, in Figure 13-1, I opened this file. If you save the file as a Photoshop.psd file, it
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Photoshop comes with many options that can be overwhelming, especially at first. As a beginner in graphic design, you might not know what all these options do and how to change them. Learning about Photoshop Elements is fairly easy, even if you’re not an expert. By the end of this guide, you will have a general understanding of the software and some of its tools. 1. Color Picker and Opacity Photoshop has a color picker, which includes many different tools to let you select colors quickly and easily. Some work much better than others. The first tool is the eyedropper tool. Here, you click in the photo or on the canvas, and then a small black dot appears. This is the color that the eyedropper is set to. If you see the color you want, click the eyedropper and it will change the color to the one you’re hovering over. You can also use the eyedropper tool to change the color without changing the image, by using the eyedropper as a tool to fill in a hole in the image with the color you chose. You can also use the eyedropper as a tool to get information about the color. In the tool window, the eyedropper’s info is shown. The pickable color is colored blue, and the hex code is shown. You can choose how the eyedropper tool behaves. The most common option is for the eyedropper to fill the color into the image with a spot, but you can choose it to paint the color as a gradient instead. You can also choose to lock the eyedropper, so the color won’t change until you choose a different color. Many people like the eyedropper with three options: one color, background color, and same color. The eyedropper tools shows up in a few places in Photoshop: on the preview and on the canvas. Here’s a screenshot of the eyedropper tool: If you see the eyedropper tool on a photo, it’s color will change to whatever you picked in the color palette and it will be in the eyedropper’s info. And here’s a screenshot of the eyedropper on the preview: You can click the eyedropper tool in the toolbox 05a79cecff
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PAM is a highly specific cell-permeable compound that has been proposed as a specific inducer of apoptosis in tumor cells. However, the potential of PAM to induce apoptosis in normal cells has not been investigated to date. We report here that PAM induces apoptosis in a variety of non-transformed cells, indicating a therapeutic potential for this compound in the treatment of diverse human diseases. MATERIALS AND METHODS {#s1} ===================== LIFESCIENCES {#s2} ———— Embryonic stem (ES) cells were cultured according to published protocols ([@BIO012670C14]) and mouse embryonic fibroblasts (MEFs) were derived from E13.5 mouse embryos. Cells were cultured in DMEM medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Life Technologies), LIF (1,000 units/ml), non-essential amino acids (1×) (Life Technologies), and 2 mmol/l glutamine (Life Technologies). Cells were maintained at 37°C in a 5% CO~2~ incubator. APOPTOSIS {#s3} ——— Immunocytochemistry was used to assess the induction of apoptosis by PAM. ES cells were cultured on poly-L-lysine (100 µg/ml) coated coverslips. Cells were treated with 20 µM PAM for 24 h, fixed in 4% paraformaldehyde and stained with rabbit anti-cleaved caspase 3 monoclonal antibody (1:1000, Cell Signaling Technology). Cells were then washed and incubated with a fluorescent labeled anti-rabbit secondary antibody (1:1000, Sigma). Nuclei were visualized using 4′,6′-diamidino-2-phenylindole (DAPI, 5 µg/ml) and cells were mounted on slides and viewed by fluorescence microscopy. A total of three independent experiments were performed. PAM treatment was also assessed by flow cytometry to detect cells that had undergone apoptosis. ES cells were trypsinized and treated with 0.5×10^6^ cells in 0.1 ml of 0.5 ml PBS with either 0 µM or 25 �
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Q: how to change item in a listbox after the application is finished from another application I have a listbox that has items displayed in it after a button is pressed in another application. I want to display a different message at the top of the listbox when the button is pressed. I know how to display a message within the application, but I am not sure how to change the contents of a listbox from another application. A: Not exactly sure what you mean by “listbox that has items displayed in it after a button is pressed in another application”, but if you are talking about ListBoxes in forms, you can access them directly from your other application via the “Windows Messages” API. Have a look at this question that uses SendMessage to access ListBox control from other application (there’s an example of using SendMessage): How to get a ListBox of an application in my current application Q: Spring 3 mvc: why is the automatic list of controllers method-scoped? I was wondering why the automatic method-scoping list of controllers method in the spring mvc pages like: it is method-scoped. I thought it should be private/final inner class of the controller, because otherwise it seems a bit strange to have only one class with all the controller methods. Edit: I’ve made a sample project here: A StackTrace of the 404 would be nice. A: This list is essentially a separate class that has a static reference to the Spring MVC annotation scan information. The Spring MVC annotation scan information is provided to all other parts of Spring MVC with an injection reference that is accessible on the class level to the Spring MVC classes. The class isn’t “package-private” because Spring MVC classes can be inherited from by Spring MVC components that are not a Spring MVC component. For example, you could have an old Spring Web MVC component that extends another Spring MVC controller (like a dispatcher). The old Spring Web MVC controller does not know about Spring MVC and therefore cannot use the Spring MVC API (it cannot invoke the controller methods since it is not a Spring
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Notes: The Pathfinder Through the Eyes of a Thief By Alexander Randolph Starting from a first-person perspective, a thief is the embodiment of a thief, an objective, foreboding force. In his hands and hands alone, the Pathfinders are a tool for doing what others are afraid of: standing up to the police. For the pathfinder, law enforcement is not an ally but an enemy. This is his fight. Whether he is caught or not, the pathfinder is determined to succeed, not for him or for the
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