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Spartacus – Wikipedia. The real-life story of Spartacus, a slave who led a slave revolt against their Roman captors.Consistently voted the best Latin party in the UK.
The weekend may have come and gone, but we’re prepared for the long summer nights that are just around the corner. We’ve got some brilliant party announcements from our friends at Club Blasé and Co, plus our favourite Spanish/Latin parties to hit in August, September and beyond. If you’re into Latin, check out the dates below, or head straight to the best social spots for Latin nights in Manchester for a night out with the most gorgeous Latin men.Arabidopsis nopaline synthase contains a nuclear localization signal targeted to the chloroplast via the transit peptide.
The bacterial type of DNA polymerase I (Nopaline Synthase, NOS) is found in several species of legume and pea (Fabaceae). Recently, the first plant NOS cDNA clone from Arbidopsis thaliana was reported. The encoded NOS polypeptide is composed of an N-terminal region that is highly similar to bacterial NOSs and a C-terminal region that is absent from bacterial NOSs. In contrast to the bacterial NOSs, the purified Arabidopsis NOS enzyme is targeted to the chloroplast. Here we report on the identification of a nucleolar localization signal within the plant NOS polypeptide. By fusing the NOS polypeptide coding sequences to the green fluorescent protein (GFP), we found that the fusion protein is targeted exclusively to the chloroplast. Analysis of Arabidopsis mutants in which the NOS translocation sequence was truncated by 14 amino acids showed that a 21 amino acid domain within the chloroplast localization signal is necessary and sufficient to target
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